thr77 login Options
thr77 login Options
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which ends up in chromosome instability, and thus could add to tumorigenesis. This protein was identified to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a critical position in selling cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, happen to be explained. Two further splice variants were claimed but thorough nucleotide sequence information and facts isn't but offered.
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Blocking peptides are peptides that bind particularly to your goal antibody and block antibody binding. These peptide usually has the epitope recognized via the antibody.
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This tutorial outlines further attainable optimization of mobile and immunoassay parameters to make certain the very best final results are...
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This guideline outlines even more possible optimization of cellular and immunoassay parameters to be certain the very best effects are...
Antibodies bound to the blocking peptide now not bind towards the epitope within the goal protein. By comparing the staining within the blocked antibody as opposed to the antibody alone, you can see which staining is unique.
Target Information Cyclin E belongs towards the remarkably conserved cyclin loved ones, whose associates are characterized by a remarkable periodicity in protein abundance throughout the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins exhibit unique expression and degradation patterns which contribute to your temporal coordination of every mitotic function. Cyclin E forms a fancy with and functions as being a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates on the G1-S phase boundary which is degraded as cells development by means check here of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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